The measurement methods of an antioxidant activity at wines

Abstract

A new method for measuring the antioxidant activity is the method which using N, N’ –dimethyl-pphenylendiamina (DMPD). The method is rapid and inexpensive, ensures sensitive and reproducibility. In this paper, was verified of there effectiveness of the DMPD method on antioxidant foods. We used wine samples coming from different areas of Romania. Antioxidant action of wines is strictly related to the amount of phenolic compounds. To evaluate the sensitivity of the method, the system was tested by using of standard solution of TROLOX 1mg/mL and DMPD: FeCl3 molar ratio of 10:1. Spectrofotometric measurements were recorded by using an UV-VIS Jenway 6300 at 505 nm. Antioxidant action was expressed as TEAC (TROLOX equivalent antioxidant capacity), using the calibration curves plated with different amounts of TROLOX. These results show that the red wine samples have a high antioxidant action, in conformed to the amount of phenolic compounds. The method ensures sensibility and reproducibility in the measurement of antioxidant action of hydrolytic compounds.