Optimization the hydrolysis process of tannic acid for gallic acid production by tannase of Aspergillus awamori using response surface methodology

Abstract

The effect of different assay conditions such as; incubation time, temperature, pH, and tannic acid concentrations on the hydrolytic efficiency of tannase from Aspergillus awamori were studied. Response surface methodology (RSM) was used in this optimization by implementing the central composite design (CCD). Statistical analysis of the results showed that linear and quadric terms of these variables had significant effects on the yield of gallic acid formation. However,
interactions between the variables were found to contribute to the response at a significant level. The predicted values of studied factors are 30.4 min incubation, 51°C, pH 5.6 and 25.3mg/mL tannic acid concentration, which resulted in 25.281μg/mL gallic acid. The resulted value of gallic acid by this way is very high as compared to the amount obtained by ‘one- at- a- time’ approach (5.20μg/mL).