Aspects regarding the profile of intestinal microbiota on wild populations of sterlet (Acipenser ruthenus, linnaeus, 1758)

  • Manuela Gianina Băcanu Dunarea de Jos University of Galati, Faculty of Food Science and Engineering
  • Lucian Oprea Dunarea de Jos University of Galati, Faculty of Food Science and Engineering
  • Georgiana Călin Sandu Dunarea de Jos University of Galati, Faculty of Food Science and Engineering
  • Rodica Dinică Dunarea de Jos University of Galati, Faculty of Food Science and Engineering
  • Marinela Maereanu Kaviar House Bucuresti Filiala Tulcea
  • Dumitru Măereanu Kaviar House Bucuresti Filiala Tulcea
  • Constanța Șerban ITCSMS Brăila
Keywords: bacterial community, sterlet, PCR, DGGE

Abstract

 The aim of the present research was to examinate the profile of intestinal microbiota of sterlet from the Danube River. Genomic DNA was extracted from each gut fish and polymerase chain reaction (PCR) was used to amplify the conserved 16S ribosomal RNA gene. Using Denaturing Gradient Gel Electrophoresis (DGGE) of PCR amplified 16S rDNA to characterise the variability of bacterial populations, the results showed different microbial profiles for 50% of fish. These results demonstrate the potential of PCRDGGE-based analysis for identification of gut microbiota and could
contribute to a better understanding of fish ecology.

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Published
2012-11-22
How to Cite
Băcanu, Manuela Gianina, Lucian Oprea, Georgiana Călin Sandu, Rodica Dinică, Marinela Maereanu, Dumitru Măereanu, and Constanța Șerban. 2012. “Aspects Regarding the Profile of Intestinal Microbiota on Wild Populations of Sterlet (Acipenser Ruthenus, Linnaeus, 1758)”. The Annals of the University Dunarea De Jos of Galati. Fascicle VI - Food Technology 36 (2), 58-63. https://gup.ugal.ro/ugaljournals/index.php/food/article/view/3414.
Issue
Vol 36 No 2 (2012)
Section
ORIGINAL ARTICLES