Optimization of biosynthesis conditions and catalitic behavior evaluation of cellulase-free xylanase produced by a new Streptomyces sp. strain

  • Gigi Coman Dunărea de Jos” University, Faculty of Food Science and Engineering, Bioengineering in Food Industry Department
  • Constantin Adina Dunărea de Jos” University, Faculty of Food Science and Engineering, Bioengineering in Food Industry Department
  • Gabriela Enache Dunărea de Jos” University, Faculty of Food Science and Engineering, Bioengineering in Food Industry Department
  • Luminița Georgescu Dunărea de Jos” University, Faculty of Food Science and Engineering, Bioengineering in Food Industry Department
  • Gabriela Bahrim Dunărea de Jos” University, Faculty of Food Science and Engineering, Bioengineering in Food Industry Department
Keywords: cellulose-free xylanase, Streptomyces, submerge fermentation, thermostable enzyme

Abstract

Cellulase-free xylanase by Streptomyces sp.P12-137 was obtained by cultivation on the wheat bran as the sole carbon source. The effect of carbon and nitrogen sources and a ratio of them on the cellulase-free xylanase production was investigated. The new isolate Streptomyces sp. strain was able to grow in submerged system and to produce an increased level of xylanase. Wheat bran induced xylanase biosynthesis yield at a high level (9.27 UA/ml). For economical reasons cultivation was achieved on a cheap
fermentative medium represented by agro-industrial wastes. The optima of
the pH and temperature of the crude xylanase activity were 5.5 and 70°C,
respectively.

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Published
2011-12-23
How to Cite
Coman, Gigi, Constantin Adina, Gabriela Enache, Luminița Georgescu, and Gabriela Bahrim. 2011. “Optimization of Biosynthesis Conditions and Catalitic Behavior Evaluation of Cellulase-Free Xylanase Produced by a New Streptomyces Sp. Strain”. The Annals of the University Dunarea De Jos of Galati. Fascicle VI - Food Technology 35 (1), 34-44. https://gup.ugal.ro/ugaljournals/index.php/food/article/view/3434.
Issue
Vol 35 No 1 (2011)
Section
ORIGINAL ARTICLES