Phytochemical and biological screening of aqueous Galium verum L. extract

  • Alexandra-Denisa Semenescu Department of Toxicology, Drug Industry, Management and Legislation, Faculty of Pharmacy, “Victor Babes” University of Medicine and Pharmacy Timisoara, 2nd Eftimie Murgu Square, 300041 Timisoara, Romania; Research Centre for Pharmaco-Toxicological Evaluation, “Victor Babes” University of Medicine and Pharmacy, 2nd Eftimie Murgu Square, 300041 Timisoara, Romania https://orcid.org/0000-0001-7718-4168
  • Elena-Alina Moacă Department of Toxicology, Drug Industry, Management and Legislation, Faculty of Pharmacy, “Victor Babes” University of Medicine and Pharmacy Timisoara, 2nd Eftimie Murgu Square, 300041 Timisoara, Romania; Research Centre for Pharmaco-Toxicological Evaluation, “Victor Babes” University of Medicine and Pharmacy, 2nd Eftimie Murgu Square, 300041 Timisoara, Romania https://orcid.org/0000-0002-2631-7028
  • Raul Chioibaş Department of Surgery I, Faculty of Medicine, “Victor Babes” University of Medicine and Pharmacy, 2nd Eftimie Murgu Square, 300041 Timișoara, Romania; CBS Medcom Hospital, 12th Popa Sapca Street, 300047 Timisoara, Romania https://orcid.org/0009-0004-1783-9109
  • Andrada Iftode Department of Toxicology, Drug Industry, Management and Legislation, Faculty of Pharmacy, “Victor Babes” University of Medicine and Pharmacy Timisoara, 2nd Eftimie Murgu Square, 300041 Timisoara, Romania; Research Centre for Pharmaco-Toxicological Evaluation, “Victor Babes” University of Medicine and Pharmacy, 2nd Eftimie Murgu Square, 300041 Timisoara, Romania https://orcid.org/0009-0007-8532-2066
  • Diana-Simona Tchiakpe-Antal Department of Pharmaceutical Botany, Faculty of Pharmacy, “Victor Babes” University of Medicine and Pharmacy Timisoara, 2nd Eftimie Murgu Square, 300041 Timisoara, Romania https://orcid.org/0000-0001-8107-8595
  • Laurian Vlase Department of Pharmaceutical Technology and Biopharmaceutics, Faculty of Pharmacy, “Iuliu Hatieganu” University of Medicine and Pharmacy, 8th Victor Babes Street, 400347 Cluj-Napoca, Romania https://orcid.org/0000-0002-0664-3387
  • Ana-Maria Vlase Department of Pharmaceutical Botany, Faculty of Pharmacy, “Iuliu Hatieganu” University of Medicine and Pharmacy, 8th Victor Babes Street, 400347 Cluj-Napoca, Romania https://orcid.org/0000-0003-4865-0777
  • Delia Muntean Department of Microbiology, Faculty of Medicine, “Victor Babes” University of Medicine and Pharmacy Timisoara, 2nd Eftimie Murgu Square, 300041 Timisoara, Romania; Multidisciplinary Research Center on Antimicrobial Resistance, “Victor Babes” University of Medicine and Pharmacy, 2nd Eftimie Murgu Square, 300041 Timisoara, Romania https://orcid.org/0000-0001-9100-4530
  • Cristina-Adriana Dehelean Department of Toxicology, Drug Industry, Management and Legislation, Faculty of Pharmacy, “Victor Babes” University of Medicine and Pharmacy Timisoara, 2nd Eftimie Murgu Square, 300041 Timisoara, Romania; Research Centre for Pharmaco-Toxicological Evaluation, “Victor Babes” University of Medicine and Pharmacy, 2nd Eftimie Murgu Square, 300041 Timisoara, Romania https://orcid.org/0000-0002-5482-7092
DOI: https://doi.org/10.35219/foodtechnology.2023.2.11
Keywords: Galium verum L., antioxidant activity, DPPH test, antimicrobial activity, cytotoxicity, melanoma

Abstract

Plants are an important source of active compounds with numerous therapeutic benefits. Nowadays, plant extracts have attracted an increased attention as candidates in the prevention and treatment of various diseases, including cancer. Galium verum L. is considered an effective remedy due to its extensive traditional use, thus the investigation of its anticancer potential has gained momentum. Malignant melanoma is an aggressive type of cancer with an increased incidence. Therefore, the purpose of this paper was to analyze the cytotoxic effect of GvH2O extract on A375 cells. Furthermore, the safety of the extract was evaluated on HaCaT cells and the results showed that the GvH2O does not exert a cytotoxic action on keratinocytes but stimulates their proliferation at 15 - 55 μg/mL. In addition, the GvH2O extract was characterized from a phytochemical point of view. The results showed that GvH2O contains polyphenolic compounds (rutin and chlorogenic acid), possesses antioxidant activity and can be effective on Gram-positive bacteria as well as on E. coli. GvH2O extract showed a cytotoxic effect on melanoma cells, causing changes in confluence and morphology and revealing nuclear characteristics specific to apoptosis. Finally, Galium verum L. may be a possible candidate for skin cancer therapy, but additional studies are needed.

Published
2023-09-08
How to Cite
Semenescu, Alexandra-Denisa, Elena-Alina Moacă, Raul Chioibaş, Andrada Iftode, Diana-Simona Tchiakpe-Antal, Laurian Vlase, Ana-Maria Vlase, Delia Muntean, and Cristina-Adriana Dehelean. 2023. “Phytochemical and Biological Screening of Aqueous Galium Verum L. Extract”. The Annals of the University Dunarea De Jos of Galati. Fascicle VI - Food Technology 47 (2), 172-89. https://doi.org/https://doi.org/10.35219/foodtechnology.2023.2.11.
Issue
Vol 47 No 2 (2023)
Section
ORIGINAL ARTICLES

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