Phytochemical screening and preliminary biological in vitro assessment of Camellia sinensis L. ethanolic extract

  • Andreea Geamantan-Sîrbu Faculty of Pharmacy, “Victor Babes” University of Medicine and Pharmacy Timisoara, 2nd Eftimie Murgu Square, Timisoara 300041, Romania; Research Centre for Pharmaco-Toxicological Evaluation, “Victor Babes” University of Medicine and Pharmacy, 2nd Eftimie Murgu Square, Timisoara 300041, Romania https://orcid.org/0009-0006-6869-9614
  • Alexandra Semenescu Faculty of Pharmacy, “Victor Babes” University of Medicine and Pharmacy Timisoara, 2nd Eftimie Murgu Square, Timisoara 300041, Romania; Research Centre for Pharmaco-Toxicological Evaluation, “Victor Babes” University of Medicine and Pharmacy, 2nd Eftimie Murgu Square, Timisoara 300041, Romania https://orcid.org/0000-0001-7718-4168
  • Alina Moacă Faculty of Pharmacy, “Victor Babes” University of Medicine and Pharmacy Timisoara, 2nd Eftimie Murgu Square, Timisoara 300041, Romania; Research Centre for Pharmaco-Toxicological Evaluation, “Victor Babes” University of Medicine and Pharmacy, 2nd Eftimie Murgu Square, Timisoara 300041, Romania http://orcid.org/0000-0002-2631-7028
  • Razvan Susan Faculty of Medicine, “Victor Babes” University of Medicine and Pharmacy Timisoara, 2nd Eftimie Murgu Square, Timisoara 300041, Romania https://orcid.org/0000-0003-3531-3974
  • Robert Jijie Faculty of Medicine, “Victor Babes” University of Medicine and Pharmacy Timisoara, 2nd Eftimie Murgu Square, Timisoara 300041, Romania https://orcid.org/0000-0002-3619-2484
  • Cristian Vlad Faculty of Medicine, “Victor Babes” University of Medicine and Pharmacy Timisoara, 2nd Eftimie Murgu Square, Timisoara 300041, Romania
Keywords: green tea, DPPH, FT-IR, colon cancer

Abstract

Camellia sinensis L. (Theaceae), is the most frequently consumed beverage worldwide, due to its human health benefits. The antioxidant compounds are known to act as a protective barrier of cell membranes against oxidative injury caused by various free radicals as well as by reactive oxygen species. Additionally, even phenolic compounds have pharmaceutical effects on oxidative injury from diverse causes. Therefore, the present study was conceived to establish the phytochemical screening, as well as the physicochemical profile of an ethanolic extract of aerial parts of Camelia sinensis L. acquired from a local authentic herbal distributor. In this respect, the total phenolic and flavonoid contents were investigated, as well as the antioxidant capacity of the green tea ethanolic extract obtained. For the physicochemical profile, the organic functional groups present in the green tea extract, and their thermal behavior were identified. Moreover, a preliminary in vitro biological assay was performed, through the evaluation of the antitumor potential of the green tea ethanolic extract obtained on the colon adenocarcinoma cell line (HT-29) compared to a healthy cell line, HaCaT (human immortalized keratinocytes). The results after the biological assessment suggest that the extract decreases the viability of HT-29 colon tumor cells (67% at a concentration of 100 µg/mL), showing a lesser effect on HaCaT cells; this being a first step in exploring the anticancer properties of the obtained extract.

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Published
2024-03-05
How to Cite
Geamantan-Sîrbu, Andreea, Alexandra Semenescu, Alina Moacă, Razvan Susan, Robert Jijie, and Cristian Vlad. 2024. “Phytochemical Screening and Preliminary Biological in Vitro Assessment of Camellia Sinensis L. Ethanolic Extract”. The Annals of the University Dunarea De Jos of Galati. Fascicle VI - Food Technology 48 (1), 163-77. https://doi.org/https://doi.org/10.35219/foodtechnology.2024.01.10.
Section
ORIGINAL ARTICLES